Use cryopreservation for setting up a bank of oil palm (Elaeis guineensis Jacq.) somatic embryos.
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Kuala Lumpur : PORIM,
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The oil palm sometic embryo cryopreservation technique which was recently developed by Engelmann and Duval (1986) ensures the regular resumption of 10 to 30% of frozen embryoids. The same results have been observed with embryoids stored for 15 months in liquid nitrogen. Plantlets obtained from frozen matieral are now growing in the IHRO/CIRAD La Mé Research Station nursery, Cote d.Ivoire. So far their development has been nornal. From now onwards cryopreservation seems applicable to the storage of embryoid strains produced by in vitro culture. The use of the process, which is reliable, inexpensive, and easy to employ will reduce the risks of variability due to in vitro culture and facilitate the management of the laboratories producing clonal plantets of oil palm.
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The oil palm sometic embryo cryopreservation technique which was recently developed by Engelmann and Duval (1986) ensures the regular resumption of 10 to 30% of frozen embryoids. The same results have been observed with embryoids stored for 15 months in liquid nitrogen. Plantlets obtained from frozen matieral are now growing in the IHRO/CIRAD La Mé Research Station nursery, Cote d.Ivoire. So far their development has been nornal. From now onwards cryopreservation seems applicable to the storage of embryoid strains produced by in vitro culture. The use of the process, which is reliable, inexpensive, and easy to employ will reduce the risks of variability due to in vitro culture and facilitate the management of the laboratories producing clonal plantets of oil palm.
13 ref.
The oil palm sometic embryo cryopreservation technique which was recently developed by Engelmann and Duval (1986) ensures the regular resumption of 10 to 30% of frozen embryoids. The same results have been observed with embryoids stored for 15 months in liquid nitrogen. Plantlets obtained from frozen matieral are now growing in the IHRO/CIRAD La Mé Research Station nursery, Cote d.Ivoire. So far their development has been nornal. From now onwards cryopreservation seems applicable to the storage of embryoid strains produced by in vitro culture. The use of the process, which is reliable, inexpensive, and easy to employ will reduce the risks of variability due to in vitro culture and facilitate the management of the laboratories producing clonal plantets of oil palm.
Palabras clave
Congelación., Elaeis guineensis., Embrión somático., Germoplasma., Preservación., Propagación vegetativa., Palma de aceite