Cloning date palm (Phoenix dactylifera L.) DNA and characterization of low, medium and high copy DNA sequences.
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Aitchitt, M.,
Ainsworth, C.C.
Mantell, S.H.
Thangavelu, M.
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Total DNA was extracted from mature leaves of the date palm (Phoenix dactylifer L.) cultivas 'Bou-Sthammi Noire". The DNA was digested with Pst 1 and the fragments shotgun-cloned in E. coli DH5Ó using the plasmid vecto pU19. A total of 124 clones were characterizad by dot blot hybridization using P labelled genomic DNA as the probe. 74% of the isolates contained single copy sequences. Many of the cloned fragments (58%) were less than 900 bp. Some of the single copy clones, when used as probes in Southern blot analysis of DNA extracted from twelve Moroccan date palm cultivars, detected restriction fragment length polymorphisms. The construction and the availability of a genomic library opens up the possibility of obtaining a panel of potentially interesting genomic DNA clones of initiating RFLP studies on breeding line of Moroccan date palms in order to speed up the selectiona and breeding of Bayoud resistant palms in Morocco.
Total DNA was extracted from mature leaves of the date palm (Phoenix dactylifer L.) cultivas 'Bou-Sthammi Noire". The DNA was digested with Pst 1 and the fragments shotgun-cloned in E. coli DH5Ó using the plasmid vecto pU19. A total of 124 clones were characterizad by dot blot hybridization using P labelled genomic DNA as the probe. 74% of the isolates contained single copy sequences. Many of the cloned fragments (58%) were less than 900 bp. Some of the single copy clones, when used as probes in Southern blot analysis of DNA extracted from twelve Moroccan date palm cultivars, detected restriction fragment length polymorphisms. The construction and the availability of a genomic library opens up the possibility of obtaining a panel of potentially interesting genomic DNA clones of initiating RFLP studies on breeding line of Moroccan date palms in order to speed up the selectiona and breeding of Bayoud resistant palms in Morocco.
Total DNA was extracted from mature leaves of the date palm (Phoenix dactylifer L.) cultivas 'Bou-Sthammi Noire". The DNA was digested with Pst 1 and the fragments shotgun-cloned in E. coli DH5Ó using the plasmid vecto pU19. A total of 124 clones were characterizad by dot blot hybridization using P labelled genomic DNA as the probe. 74% of the isolates contained single copy sequences. Many of the cloned fragments (58%) were less than 900 bp. Some of the single copy clones, when used as probes in Southern blot analysis of DNA extracted from twelve Moroccan date palm cultivars, detected restriction fragment length polymorphisms. The construction and the availability of a genomic library opens up the possibility of obtaining a panel of potentially interesting genomic DNA clones of initiating RFLP studies on breeding line of Moroccan date palms in order to speed up the selectiona and breeding of Bayoud resistant palms in Morocco.
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Adn., Clones., Dátiles, Genética., Genomas., Palma datilera