Immunological study of Rubisco in oil palm and coconut. - (:unkn) unknown (My University)
for both types of material.
in those of adult palms. For coconut, the figure was around 25.
of total soluble proteins in the leaves of oil palm ramets and 35.
Ribulose1,5bisphosphate carboxylase/oxygenase (RubisCO; EC 4.1.1.39) was studied in the leaves of oil palm and coconut in vitro plantlets and adult plants. The Ouchterlony double immunodiffusion test was applied to the enzyme extracts of both species using rabbit antisera raised against RubisCO from spinach, tobacco and maize. To quantify RubisCO in the material sampled from adult palms and in vitro plantlets, an estimation method was developed based on the Laurell immunoelectrophoresis test. TheRubisCO contents measured represented around 25.
Keywords: biochemistry; Coconut; composition; determination; enzymes; immunological techniques; in vitro culture; leaves; oil palms; oilseed plants; ribulose bisphosphate carboxylase; tropical crops; Biochemistry; Biochemistry; Biochemistry; Coconut; Composition; Enzymes; Enzymes; Leaves; Leaves; Oilseed plants; Tropical crops