Synthesis of Triacylglycerol Containing Conjugated Linoleic Acid by Esterification Using Two Blended Lipases. - (:unkn) unknown (My University)
We have developed an efficient esterification for the synthesis of triacylglycerol (TAG) containing conjugated linoleic acids (CLA) using a blend of two powdered lipases. Two pairs of blended lipases promoted the esterification. Rhizomucor miehei lipase plus Alcaligenes sp. lipase and Penicillium camembertii MAG and DAG lipase plus Alcaligenes sp. lipase were used. At the optimal ratio of two lipases, the content of TAG containing CLA (TAG-CLA) in all glycerols reached 82-83 percent after 47 h using 1 wt percent of lipases. With R. miehei lipase plus Alcaligenes sp. lipase, the reaction time to obtain ca. 60 percent of TAG-CLA was one-third of that needed with R. miehei lipase alone. The optimal ratio of two lipases differed between these two pairs. The optimal ratio was 70-80 wt percent of R. miehei lipase in the last stage of the reaction, whereas it was over a wide range of 10-90 wt percent for P. camembertii lipase. In the blend of R. miehei lipase plus Alcaligene sp. lipase, activity remained very high after 10 cycles of esterification (every 47 h) and could be used in the industrial production of TAG-CLA.
We have developed an efficient esterification for the synthesis of triacylglycerol (TAG) containing conjugated linoleic acids (CLA) using a blend of two powdered lipases. Two pairs of blended lipases promoted the esterification. Rhizomucor miehei lipase plus Alcaligenes sp. lipase and Penicillium camembertii MAG and DAG lipase plus Alcaligenes sp. lipase were used. At the optimal ratio of two lipases, the content of TAG containing CLA (TAG-CLA) in all glycerols reached 82-83 percent after 47 h using 1 wt percent of lipases. With R. miehei lipase plus Alcaligenes sp. lipase, the reaction time to obtain ca. 60 percent of TAG-CLA was one-third of that needed with R. miehei lipase alone. The optimal ratio of two lipases differed between these two pairs. The optimal ratio was 70-80 wt percent of R. miehei lipase in the last stage of the reaction, whereas it was over a wide range of 10-90 wt percent for P. camembertii lipase. In the blend of R. miehei lipase plus Alcaligene sp. lipase, activity remained very high after 10 cycles of esterification (every 47 h) and could be used in the industrial production of TAG-CLA.
Keywords: Ácido linoléico.; Esterificación.; Lipasa; Triacilglicerol