The influence of low temperature treatment on growth and poline accumulation in plyembryogenic cultures of oil palm (Elaeis guineensis Jacq.). - (:unkn) unknown
Low temperatures (15C-20C) were observed to inhibit the growth and multiplication of oil palm polyembriogenic (PE) cultures. These conditions enable PE cultures to be kept for at least six months without subculturing. The method can, therefore, be exploited for minimal growth storage of in vitro cultures. Proline, which is known to be an universal stress indicatyor, was found to accumulate in the cultures under low temperature stress and the increase was about two-fold during 30 days of exposure. In the presence of 0.5 M surcrose, low temperature storage of cultures could be extended even to nine months.
Low temperatures (15C-20C) were observed to inhibit the growth and multiplication of oil palm polyembriogenic (PE) cultures. These conditions enable PE cultures to be kept for at least six months without subculturing. The method can, therefore, be exploited for minimal growth storage of in vitro cultures. Proline, which is known to be an universal stress indicatyor, was found to accumulate in the cultures under low temperature stress and the increase was about two-fold during 30 days of exposure. In the presence of 0.5 M surcrose, low temperature storage of cultures could be extended even to nine months.
Keywords: Cultivo de tejidos.; Cultivo in vitro.; Elaeis guineensis.; Prolina.; Temperatura.; Palma de aceite