Long-Term in vitro maintenance of oil palm (Elaeis guineensis Jacq.) Clones through axenic root cultures. - (:unkn) unknown
Studies were carried out to determine whether oil palm clones could be maintained long-term in vitro by means of root cultures. It was found that isolated root explants excised from axenic ramets could be maintained in culture for at least 18 months by repeated subsculture. Root explants 3 cms in length procuded more axillary roots than 1 cm root explants. Root growth was best in basal liquid medium containing mofified MS nutrients supplemented with 2.5 g.L-1 of activated charcoal. A full strength modified MS medium supported root tip elongation and production of axillary roots better than a half strength nutrient mediu. root explants with root tips (RT) multiplied by root tip elongation and production of axillary roots whilst roots devoid of root tip (RS) multiplied by production of axillary roots only. Root tip segments (RT) sustained growth better than RS segments during subsculturing, but there was a decline in the growth vigour of the new explants in the subsequent subcultures.
Studies were carried out to determine whether oil palm clones could be maintained long-term in vitro by means of root cultures. It was found that isolated root explants excised from axenic ramets could be maintained in culture for at least 18 months by repeated subsculture. Root explants 3 cms in length procuded more axillary roots than 1 cm root explants. Root growth was best in basal liquid medium containing mofified MS nutrients supplemented with 2.5 g.L-1 of activated charcoal. A full strength modified MS medium supported root tip elongation and production of axillary roots better than a half strength nutrient mediu. root explants with root tips (RT) multiplied by root tip elongation and production of axillary roots whilst roots devoid of root tip (RS) multiplied by production of axillary roots only. Root tip segments (RT) sustained growth better than RS segments during subsculturing, but there was a decline in the growth vigour of the new explants in the subsequent subcultures.
Keywords: Clones.; Cultivo de tejidos.; Medio de cultivo.; Raíz; Palma de aceite