Infection of Oryctes rhinoceros L. by application of Metarhizium anisopliae (Metsch.) Sorokin to breeding sites. - (:unkn) unknown
A preliminary experiment was carried out in the Lower Perak district of Malaysia on the field application of Metarhizium anisopliae for controlling Oryctes rhinoceros in a 2-year-old oil palm replant. Spores of M. anisopliae were mass produced on maize grains by inoculating a spore suspension of the fungus into 500 g autoclaved batches of the medium in polypropylene bags. Ten bags of the inoculum were applied to 10 m x 3 m heaps of rotting coconut debris in the oil palm planting. Treatments consisted of monthly application for 2 or 3 months. Control plots were left untreated. Treatments were replicated 4 times in a completely randomized design. Application of Metarhizium inoculum to Oryctes breeding sites increased the incidence of infection by the fungus, with 5, 42 and 83% infection of the Oryctes population 1, 3 and 4 months after treatment, resp. The frequency of application of inoculum also had an effect on the incidence of infection, with 100, 88 and 71% infection for 1, 2 and 3 applications after 4 months.
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A preliminary experiment was carried out in the Lower Perak district of Malaysia on the field application of Metarhizium anisopliae for controlling Oryctes rhinoceros in a 2-year-old oil palm replant. Spores of M. anisopliae were mass produced on maize grains by inoculating a spore suspension of the fungus into 500 g autoclaved batches of the medium in polypropylene bags. Ten bags of the inoculum were applied to 10 m x 3 m heaps of rotting coconut debris in the oil palm planting. Treatments consisted of monthly application for 2 or 3 months. Control plots were left untreated. Treatments were replicated 4 times in a completely randomized design. Application of Metarhizium inoculum to Oryctes breeding sites increased the incidence of infection by the fungus, with 5, 42 and 83% infection of the Oryctes population 1, 3 and 4 months after treatment, resp. The frequency of application of inoculum also had an effect on the incidence of infection, with 100, 88 and 71% infection for 1, 2 and 3 applications after 4 months.
Keywords: Control biológico; Control de plagas.; Hongos entomopatogenos.; Insectos dañinos.; Malaysia; Metarhizium.; Oryctes.; Palma de aceite