| dc.creator | PIPOC 2007 International Palm Oil Congress Palm oil: Empowering change
August 26-30, 2007 :
Kuala Lumpur :
52116. | |
| dc.creator | Cullis, Christopher A.
47104. | |
| dc.creator | Cullis, Margaret A.
47105. | |
| dc.creator | Meilina Ong Abdullah
47106. | |
| dc.creator | Malaysian Palm Oil Board, MPOB
50178. | |
| dc.date | 2007. | |
| dc.description | The molecular basis of somaclonal variation is not known. However, evidence points towards the existence of labile portions of the genome that can be modulated when the cells undergo the stress of tissue culture. Based on these observations a reasonable conclusion is that a set of variable sequences associated with the occurrence of somaclonal variation can be identified and used for the early detection of genome instability. This hypothesis, that there are identifiable, predictable markers diagnostic of somaclonal variation has been tested in oil palms. Representational difference analysis (RDA) was used to isolate DNA differences between culture-induced off-types and normal oil palm plants. The difference products were cloned, sequenced and then used to design PCR primers. These primers were used to compare the DNAs isolated from normal, mantled, revertant and uncultured oil palm plants. The results are consistent with the hypothesis that there is a particularly labile portion of the genome. Many of the RDA difference products highlight differences between plants regenerated from culture. Additionally since many of the primer pairs also distinguish between the different individual plants, irrespective of their phenotype, this labile fraction of the genome is also an excellent source of molecular markers. A continued characterization of this fraction of the genome that is especially susceptible to stress, with resulting higher rearrangement frequencies and mutation rates than other portions of the genome, will result in the identification of the markers useful for determining the stability of the genome as a whole, not just those responsible for somaclonal variation, and the mechanisms by which this region of the genome rapidly reorganizes. | |
| dc.description | Incluye referencias bibliográficas. | |
| dc.description | The molecular basis of somaclonal variation is not known. However, evidence points towards the existence of labile portions of the genome that can be modulated when the cells undergo the stress of tissue culture. Based on these observations a reasonable conclusion is that a set of variable sequences associated with the occurrence of somaclonal variation can be identified and used for the early detection of genome instability. This hypothesis, that there are identifiable, predictable markers diagnostic of somaclonal variation has been tested in oil palms. Representational difference analysis (RDA) was used to isolate DNA differences between culture-induced off-types and normal oil palm plants. The difference products were cloned, sequenced and then used to design PCR primers. These primers were used to compare the DNAs isolated from normal, mantled, revertant and uncultured oil palm plants. The results are consistent with the hypothesis that there is a particularly labile portion of the genome. Many of the RDA difference products highlight differences between plants regenerated from culture. Additionally since many of the primer pairs also distinguish between the different individual plants, irrespective of their phenotype, this labile fraction of the genome is also an excellent source of molecular markers. A continued characterization of this fraction of the genome that is especially susceptible to stress, with resulting higher rearrangement frequencies and mutation rates than other portions of the genome, will result in the identification of the markers useful for determining the stability of the genome as a whole, not just those responsible for somaclonal variation, and the mechanisms by which this region of the genome rapidly reorganizes. | |
| dc.language | ng | |
| dc.publisher | Kuala Lumpur : MPOB, | |
| dc.subject | Palma de aceite | |
| dc.title | Development of markers for the mantled phenotype (and somaclonal variants in general) in oil palm. | |
| dc.type | text | |
