Distribution of fatty acids in internal and external positions of triacylglycerols in 2 cyclopropanoid oils : litchi and longan.
Author
Grondin, I.,
autor.
aut
56461
Farines, M.
42357
Smadja, J.
56462
Soulier, J.
42358
Como citar
Abstract
Triacylglycerols in Litchi sinensis [L. chinensis] and longan (Euphoria longana [Dimocarpus longan]) seed oils are characterized by cyclopropanoic fatty acids: cis-9,10-methyleneoctadecanoic acid or dihydrosterculic acid constitutes 38.0% of total fatty acids in litchi and 17.4% in longan, whereas cis-7,8-methylenehexadecanoic acid only constitutes 5.5% and 1.0%, respectively. Triacylglycerols of these 2 oils underwent enzymatic hydrolysis with porcine pancreatic lipase or chemical degradation withethyl magnesium bromide, a reagent without chain specificity or regioselectivity. An unequal distribution of cis-7,8-methylenehexadecanoic and cis-9,10-methyleneoctadecanoic acids was found. The former was found in the sn-2 position at 84% in litchi triacylglycerols and at 100% in longan triacylglycerols, while the latter was located in external positions at 88% and 92%, respectively. Comparison of results from the 2 methods confirmed the impossibility of using the results obtained from enzymatic hydrolysis to determine the internal and external distribution of fatty acids in triacylglycerols containing cyclopropanoic fatty acids. Triacylglycerols in Litchi sinensis [L. chinensis] and longan (Euphoria longana [Dimocarpus longan]) seed oils are characterized by cyclopropanoic fatty acids: cis-9,10-methyleneoctadecanoic acid or dihydrosterculic acid constitutes 38.0% of total fatty acids in litchi and 17.4% in longan, whereas cis-7,8-methylenehexadecanoic acid only constitutes 5.5% and 1.0%, respectively. Triacylglycerols of these 2 oils underwent enzymatic hydrolysis with porcine pancreatic lipase or chemical degradation withethyl magnesium bromide, a reagent without chain specificity or regioselectivity. An unequal distribution of cis-7,8-methylenehexadecanoic and cis-9,10-methyleneoctadecanoic acids was found. The former was found in the sn-2 position at 84% in litchi triacylglycerols and at 100% in longan triacylglycerols, while the latter was located in external positions at 88% and 92%, respectively. Comparison of results from the 2 methods confirmed the impossibility of using the results obtained from enzymatic hydrolysis to determine the internal and external distribution of fatty acids in triacylglycerols containing cyclopropanoic fatty acids.
Triacylglycerols in Litchi sinensis [L. chinensis] and longan (Euphoria longana [Dimocarpus longan]) seed oils are characterized by cyclopropanoic fatty acids: cis-9,10-methyleneoctadecanoic acid or dihydrosterculic acid constitutes 38.0% of total fatty acids in litchi and 17.4% in longan, whereas cis-7,8-methylenehexadecanoic acid only constitutes 5.5% and 1.0%, respectively. Triacylglycerols of these 2 oils underwent enzymatic hydrolysis with porcine pancreatic lipase or chemical degradation withethyl magnesium bromide, a reagent without chain specificity or regioselectivity. An unequal distribution of cis-7,8-methylenehexadecanoic and cis-9,10-methyleneoctadecanoic acids was found. The former was found in the sn-2 position at 84% in litchi triacylglycerols and at 100% in longan triacylglycerols, while the latter was located in external positions at 88% and 92%, respectively. Comparison of results from the 2 methods confirmed the impossibility of using the results obtained from enzymatic hydrolysis to determine the internal and external distribution of fatty acids in triacylglycerols containing cyclopropanoic fatty acids.
Palabras clave:
enzymes
fatty acids
fatty oils
fruit crops
hydrolysis
longans
methodology
position
seed oils
seeds
subtropical fruits
triacylglycerols
Enzymes
Enzymes
Fatty acids
Hydrolysis
Methodology
Methodology
Seeds
Seeds
Tropical fruit
enzymes
fatty acids
fatty oils
fruit crops
hydrolysis
longans
methodology
position
seed oils
seeds
subtropical fruits
triacylglycerols
Enzymes
Enzymes
Fatty acids
Hydrolysis
Methodology
Methodology
Seeds
Seeds
Tropical fruit