Implementation of a test for resistance to Plasmopara halstedii on sunflower plantlets derived from in vitro culture of immature embryos.
Author
Pelletier, C.,
autor.
aut
56494
Tourvieille, de Labrouhe D.
42501
Vear, F.
42502
Como citar
Abstract
Immature embryo culture for rapid fixation of inbred lines is of particular interest if selection is possible at the same time. A methodology is presented for determining downy mildew resistance in plants from immature embryos. All concentrations between 5000 and 500 000 zoosporangia/ml gave infection levels close to 100%. Seedling age was important. At the cotyledon stage 3% of plants escaped infection, while with 1-2 pairs of true leaves 99.5% of plants were infected. Passage in a humid chamber for 48 h promoted infection. The best method was to spray plants having one pair of true leaves with a suspension of 500 000 zoosporangia/ml and keep them for 48 h in a saturated atmosphere. A breeding programme is proposed to introduce downy mildew resistance and cytoplasmic male sterility using in vitro culture. 14 ref. Immature embryo culture for rapid fixation of inbred lines is of particular interest if selection is possible at the same time. A methodology is presented for determining downy mildew resistance in plants from immature embryos. All concentrations between 5000 and 500 000 zoosporangia/ml gave infection levels close to 100%. Seedling age was important. At the cotyledon stage 3% of plants escaped infection, while with 1-2 pairs of true leaves 99.5% of plants were infected. Passage in a humid chamber for 48 h promoted infection. The best method was to spray plants having one pair of true leaves with a suspension of 500 000 zoosporangia/ml and keep them for 48 h in a saturated atmosphere. A breeding programme is proposed to introduce downy mildew resistance and cytoplasmic male sterility using in vitro culture.
Immature embryo culture for rapid fixation of inbred lines is of particular interest if selection is possible at the same time. A methodology is presented for determining downy mildew resistance in plants from immature embryos. All concentrations between 5000 and 500 000 zoosporangia/ml gave infection levels close to 100%. Seedling age was important. At the cotyledon stage 3% of plants escaped infection, while with 1-2 pairs of true leaves 99.5% of plants were infected. Passage in a humid chamber for 48 h promoted infection. The best method was to spray plants having one pair of true leaves with a suspension of 500 000 zoosporangia/ml and keep them for 48 h in a saturated atmosphere. A breeding programme is proposed to introduce downy mildew resistance and cytoplasmic male sterility using in vitro culture.
Palabras clave:
Biotecnología.
cytoplasmic male sterility
disease resistance
fatty oil plants
in vitro selection
plant breeding methods
plant diseases
plant pathogenic fungi
plant pathogens
plant pathology
sunflowers
techniques
testing
tissue culture
Cytoplasmic male sterility
Natural immunity
Plant diseases
Plant diseases
Phytopathogenic microorganisms
Plant diseases
Plant diseases
Sunflowers
Testing
Testing
Tissue culture
Biotecnología.
cytoplasmic male sterility
disease resistance
fatty oil plants
in vitro selection
plant breeding methods
plant diseases
plant pathogenic fungi
plant pathogens
plant pathology
sunflowers
techniques
testing
tissue culture
Cytoplasmic male sterility
Natural immunity
Plant diseases
Plant diseases
Phytopathogenic microorganisms
Plant diseases
Plant diseases
Sunflowers
Testing
Testing
Tissue culture
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