Implementation of a test for resistance to Plasmopara halstedii on sunflower plantlets derived from in vitro culture of immature embryos.
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Immature embryo culture for rapid fixation of inbred lines is of particular interest if selection is possible at the same time. A methodology is presented for determining downy mildew resistance in plants from immature embryos. All concentrations between 5000 and 500 000 zoosporangia/ml gave infection levels close to 100%. Seedling age was important. At the cotyledon stage 3% of plants escaped infection, while with 1-2 pairs of true leaves 99.5% of plants were infected. Passage in a humid chamber for 48 h promoted infection. The best method was to spray plants having one pair of true leaves with a suspension of 500 000 zoosporangia/ml and keep them for 48 h in a saturated atmosphere. A breeding programme is proposed to introduce downy mildew resistance and cytoplasmic male sterility using in vitro culture.
14 ref.
Immature embryo culture for rapid fixation of inbred lines is of particular interest if selection is possible at the same time. A methodology is presented for determining downy mildew resistance in plants from immature embryos. All concentrations between 5000 and 500 000 zoosporangia/ml gave infection levels close to 100%. Seedling age was important. At the cotyledon stage 3% of plants escaped infection, while with 1-2 pairs of true leaves 99.5% of plants were infected. Passage in a humid chamber for 48 h promoted infection. The best method was to spray plants having one pair of true leaves with a suspension of 500 000 zoosporangia/ml and keep them for 48 h in a saturated atmosphere. A breeding programme is proposed to introduce downy mildew resistance and cytoplasmic male sterility using in vitro culture.
14 ref.
Immature embryo culture for rapid fixation of inbred lines is of particular interest if selection is possible at the same time. A methodology is presented for determining downy mildew resistance in plants from immature embryos. All concentrations between 5000 and 500 000 zoosporangia/ml gave infection levels close to 100%. Seedling age was important. At the cotyledon stage 3% of plants escaped infection, while with 1-2 pairs of true leaves 99.5% of plants were infected. Passage in a humid chamber for 48 h promoted infection. The best method was to spray plants having one pair of true leaves with a suspension of 500 000 zoosporangia/ml and keep them for 48 h in a saturated atmosphere. A breeding programme is proposed to introduce downy mildew resistance and cytoplasmic male sterility using in vitro culture.
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Biotecnología., cytoplasmic male sterility, disease resistance, fatty oil plants, in vitro selection, plant breeding methods, plant diseases, plant pathogenic fungi, plant pathogens, plant pathology, sunflowers, techniques, testing, tissue culture, Cytoplasmic male sterility, Natural immunity, Plant diseases, Plant diseases, Phytopathogenic microorganisms, Plant diseases, Plant diseases, Sunflowers, Testing, Testing, Tissue culture